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5 DISCUSSION 51 Tissue-Engineered Vascular Grafts In this chapter we have described a biodegradable scaffold-based method of tissue engineering blood vessels with mechanical and functional properties that approach the requisite properties for clinical application Our constructs, grown under pulsatile conditions, possess many of the physiological and mechanical characteristics of native arteries, including burst strengths of more than 2000 mmHg provided entirely by the medial layer As our technique also emphasizes the creation of a functional endothelium, we have been the rst to implant autologous tissueengineered arteries that have remained patent and functional for up to 4 weeks Despite these exciting and promising accomplishments, many hurdles remain on the road to creating a fully functional tissue-based vascular graft The design of a functional nerve and microvascular supply in vitro to support the vascular tissue remains elusive.

Remove the aggregates/organoids by pouring the remaining vessel contents ( 35 ml) through the central lling port into a 50-ml centrifuge tube.

Although perhaps not an essential requirement for vascular conduits, achieving their production would mark a signi cant leap forward in the eld of tissue engineering 52 Genetic Manipulation in Tissue-Engineered Blood Vessels To create functional vascular grafts that mimic the native vasculature as closely as possible, the in vitro loss of optimal cell phenotype will need to be addressed Endothelial cells in culture, for example, are known to downregulate the expression of many anticoagulant and anti-in ammatory molecules that are expressed on the native, quiescent endothelium [Wu and Thiagarajan, 1996] Unfortunately, many procoagulant and proin ammatory molecules including tissue factor, vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) are instead upregulated during culture [Carlos and Harlan, 1994; Lin et al, 1997; Allen et al, 1998].

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Thus it appears likely that cultured endothelial cells seeded onto the lumen of tissue-engineered blood vessels exhibit a procoagulant rather than antithrombogenic phenotype, ultimately contributing to graft failure due to thrombosis Not unexpectedly, the critical limitation in the successful.

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Remark If the channel is used without feedback [i.e., if the input symbols do not depend on the past output symbols, namely, p(xk |x k 1 , y k 1 ) = p(xk |x k 1 )], the channel transition function for the nth extension of the discrete memoryless channel reduces to

Traditionally, the main emphasis in cellular networks has been on bidirectional point-to-point communication However, the bene ts that come with point-to-multipoint (p-t-m) model have been noted and therefore some steps towards this direction have been taken in 3GPP (Third-Generation Partnership Project) In release 99 of the 3GPP standards, two p-t-m concepts have already been de ned: cell broadcast service (CBS) and IP multicast support CBS enables transmission of low-bit-rate data services to a prede ned set of cells over a p-t-m bearer on the air interface [34] Because of bit rate limitations, CBS is not suitable for delivering multimedia types of data, but ts well for services whose purpose is to be available to all subscribers in a certain area and does not require high bit rates As the term states, IP multicast support enables the subscribers to receive IP multicast traf c over 3G networks [35].

In contrast to CBS, IP multicast support does not limit the transmitted data types, but enables transmission of any kind of data that can be carried over IP The major drawback in this concept is that in reality the data are always delivered over dedicated p-t-p bearers Thus, from a resource usage point of view, the data delivered using IP multicast support does not differ from normal packet calls, and no real savings can be found, so, in other words, this has no gain (a multicast gain of one) To overcome these weaknesses in the existing p-t-m services, 3GPP launched a standardization process for a new service concept Multimedia broadcast/multicast service (MBMS) is a new p-t-m bearer service that enables ef cient unidirectional p-t-m multimedia data delivery to mobile subscribers [36] MBMS has two modes of operation: broadcast mode and multicast mode.

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